螢光標定之抗體提供研究者在偵測細胞表面抗原或是胞內組成物時極高的敏感度與專一性;此項產品適用於流式細胞儀,螢光原位雜交,螢光顯微鏡影像分析以及免疫分析等實驗。SeraCare KPL提供多種標定有螢光物質之抗體以供研究者直接目視螢光分析,包括FITC,TRITC,R-phycoerythrin。
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Excitation and Emission Table
Fluorochrome | Color Emission | Excitation Wavelength | Emission Wavelength |
Fluorescein (FITC) | Green | 495 nm | 528 nm |
Rhodamine (TRITC) | Red | 555 nm | 580 nm |
R-Phycoerythrin (RPE) | Orange | 564 nm | 575 nm |
Texas Red | Red | 596 nm | 620 nm |
Cy2 | Green | 489 nm | 505 nm |
Cy3 | Orange | 552 nm | 570 nm |
Cy5 | Far-Red | 650 nm | 670 nm |
PBXL-1 | Red | 545 nm | 666 nm |
PBXL-3 | Red | 614 nm | 662 nm |
Fluorochrome | Color | Ex/Em | Replaces | Anti Human IgG | Anti Mouse IgG | Anti Rabbit IgG | Streptavidin |
DyLight405 | Blue | 400/420 | Alexa 405 & Cascade | #5230-0349-0.1mg | - | #5270-0027-1mg | |
#5230-0420-1 mg | |||||||
FITC | Green | 493/518 | Alexa 488 | #5230-0425-2mg | #5230-0427-2mg | #5230-0426-2mg | #5270-0028-1mg |
DyLight488 | Green | 493/518 | Alexa 488 & FITC | #5230-0382-1 mg | #5230-0341-0.1mg | #5230-0339-0.1mg | #5270-0017-0.1mg |
#5230-0391-1mg | #5230-0385-1mg | #5270-0024-1mg | |||||
Cy3 | Yellow | 550/568 | TRITC | #5230-0357-1mg | #5230-0364-1mg | #5230-0359-1mg | #5270-0022-1mg |
Cy5 | Red | 646/674 | Alexa 647 | #5230-0370-1mg | #5230-0376- 1mg | #5230-0372-1mg | #5270-0023-1mg |
DyLight680 | Near Infrared | 682/715 | Alexa 680 & Cy5.5 | #5230-0400-1mg | #5230-0344-0.1mg | #5230-0343-0.1mg | #5270-0025-1mg |
#5230-0406-1mg | #5230-0402-1mg | ||||||
DyLight800 | Infrared | 770/794 | IRDye 800 | #5230-0410-1mg | #5230-0347-0.1mg | #5230-0412-1mg | #5270-0026-1mg |
#5230-0415-1mg |