trc20交易平台

產(chǎn)品及服務(wù)

Blotting Kits and reagents 墨點(diǎn)法相關(guān)試劑
Western Blotting;HRP;AP;TMB;ECL;KPL;wash buffer

Blotting Substrates and Reagents

西方墨點(diǎn)法呈色劑與相關(guān)試劑

產(chǎn)品說(shuō)明
SeraCare KPL發(fā)展出專門適合於PVDF和NC membrane使用的液狀化學(xué)呈色與冷光呈色試劑；具有方便、穩(wěn)定、濃度範(fàn)圍廣泛與敏感度高等特性。
功能說(shuō)明
? ? ? ?免疫分析中Substrates是detection和呈色步驟最重要的決定因子；如何選擇適當(dāng)?shù)腟ubstrates，則可依據(jù)對(duì)敏感度的需求、不同的儀器與濾鏡求、以及酵素的選擇。酵素系統(tǒng)主要為HRP和AP系統(tǒng)。。HRP的酵素反應(yīng)催化時(shí)間比AP來(lái)的快，在短時(shí)間內(nèi)可以反應(yīng)較多的受質(zhì)，因此短時(shí)間內(nèi)的敏感度很高；AP反應(yīng)的優(yōu)點(diǎn)之一，就是AP與其受質(zhì)（substrates）的反應(yīng)呈現(xiàn)一線性反應(yīng)，所以其敏感度可隨著受質(zhì)反應(yīng)時(shí)間延長(zhǎng)而提升。研究者可依其需求來(lái)選擇適當(dāng)?shù)膕ubstrates。SeraCare KPL發(fā)展出專門適合於PVDF和NC membrane使用的液狀化學(xué)呈色與冷光呈色試劑；具有方便、穩(wěn)定、濃度範(fàn)圍廣泛與敏感度高等特性。


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HRP Membrane Substrates
Cat. # Product Name Size
5420-0020
(50-73-00)		 4CN 2-Component Membrane Peroxidase Substrate Kit 600 mL
5420-0024
(50-73-04)		 4CN 2-Component Membrane Peroxidase Substrate Kit 2.7 L
5430-0040
(54-61-00)		 LumiGLO Peroxidase Chemiluminescent Substrate Kit 240 mL
5430-0041
(54-61-01)		 LumiGLO Peroxidase Chemiluminescent Substrate Kit 720 mL
5430-0042
(54-61-02)		 LumiGLO Peroxidase Chemiluminescent Substrate Kit 60 mL
5430-0049
(54-71-00)		 LumiGLO Reserve Chemiluminescent Substrate Kit 2400 cm2
5430-0050
(54-71-01)		 LumiGLO Reserve Chemiluminescent Substrate Kit 600 cm2
5420-0027
(50-77-03)		 TMB 1-Component Membrane Peroxidase Substrate 200 mL
5420-0028
(50-77-04)		 TMB 1-Component Membrane Peroxidase Substrate 1 L
5420-0029
(50-77-18)		 TMB 1-Component Membrane Peroxidase Substrate 100 mL
5420-0026
(50-77-01)		 TMB Membrane Enhancer 40 mL
5420-0025
(50-77-00)		 TMB Membrane Peroxidase Substrate System 440 mL
AP Membrane Substrates
Cat. # Product Name Size
5420-0033
(50-81-07)		 BCIP/NBT 1-Component Phosphatase Substrate 600 mL
5420-0036
(50-81-10)?		 BCIP/NBT 1-Component Phosphatase Substrate 1 L
5420-0038
(50-81-18)		 BCIP/NBT 1-Component Phosphatase Substrate 100 mL
5420-0030
(50-81-00)		 BCIP/NBT Phosphatase Substrate System 300 mL
5430-0054
(55-60-03)		 PhosphaGLO AP Substrate 30 mL
5430-0055
(55-60-04)		 PhosphaGLO AP Substrate 100 mL
5430-0052
(55-60-01)		 PhosphaGLO Reserve AP Substrate 30 mL
5430-0053
(55-60-02)		 PhosphaGLO Reserve AP Substrate 100 mL

另外，岑祥也提供您其他在Blotting時(shí)可使用的試劑；包括 blocking, and washing steps of the assay, and?antibody conjugate stabilizers and substrate stop solutions：

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Cat. # Product Name Size
5960-0021
(50-86-05)		 20X SSC 1 L
5960-0015
(50-63-06)		 Biotin Wash Kit 200 mL
5150-0008
(50-63-00)		 Wash Solution Concentrate Kit 800 mL
5140-0006
(50-61-00)		 10% BSA Diluent/Blocking Solution 200 mL
5140-0008
(50-61-10)		 10% BSA Diluent/Blocking Solution 1 L
5560-0007
(71-00-27)		 10% Normal Goat Serum 50 mL
5560-0010
(71-18-01)		 10% Normal Mouse Serum 10 mL
5560-0008
(71-00-28)		 10% Normal Rabbit Serum 50 mL
5920-0004
(71-83-00)		 5X Detector Block 240 mL
5560-0006
(71-00-10)		 Blocking Solution Concentrate 10 mL
5140-0011
(50-82-01)		 Milk Diluent/Blocking Concentrate Kit 200 mL
5560-0009
(71-00-61)		 Universal Block for Immunohistochemistry 100 mL
5290-0007
(55-15-00)		 AP Stabilizer 200 mL
5290-0005
(54-15-01)		 HRP Stabilizer 200 mL
5120-0058
(50-80-02)		 DEA Buffer Solution 100 mL
5570-0005
(71-00-16)		 Fluorescent Mounting Media 15 mL
5450-0012
(50-22-02)		 Silver Enhancer Kit for Membranes 500 mL
5520-0021
(50-22-01)		 Silver Enhancer Kit for Microscopy 50 mL
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